| 產(chǎn)品說(shuō)明 |
成纖維細(xì)胞是間葉細(xì)胞，它在成熟的生物體發(fā)育過(guò)程中具有許多重要的功能。成纖維細(xì)胞與結(jié)締組織中細(xì)胞外基質(zhì)的合成有關(guān)，并且在創(chuàng)傷愈合過(guò)程中起著重要作用。許多疾病都與成纖維細(xì)胞有關(guān)，不僅因?yàn)槌衫w維細(xì)胞與病原論有關(guān)，而且纖維癥伴隨著組織中其它細(xì)胞類型的損害。成纖維細(xì)胞是最易獲得的哺乳動(dòng)物細(xì)胞類型而且最適于體外培養(yǎng)。牙齦成纖維細(xì)胞是牙齦組織中的主要成分，并在維持牙齦健康中起著關(guān)鍵作用。人牙齦纖維原細(xì)胞表達(dá)廣泛的表面分子，主要包括CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99和CD117。也有證據(jù)表明牙齦成纖維細(xì)胞為蛋白酶激活受體1和蛋白酶激活受體3表達(dá)mRNA。 人牙齦成纖維細(xì)胞(HGF)提取于人牙齦組織，原代凍存。每管含有細(xì)胞數(shù)>5×105 cells/ml，此細(xì)胞通過(guò)Fibronectin免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。 |
| 參考文獻(xiàn) |
1.) Herath TDK, Darveau RP, Seneviratne CJ, Wang C, Wang Y, Jin L. (2013) "Tetra- and penta-acylated lipid A structures of porphyromonas gingivalis LPS differentially activate TLR4-mediated NF-B signal transduction cascade and immuno-inflammatory response in human gingival fibroblasts." PLoS One. 8: e58496. 2.) Herath TDK, Wang Y, Seneviratne CJ, Darveau RP, Wang C, Jin L. (2013) "The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts." BMC Microbiol. 13: 73. |
