Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.32; HindIII--0.79; PvuII--1.29. Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644). Detects a transcript of 2.7 kb in lymphoid cells. |
