| Insert |
DNA: cDNA Insert lengths(kb): 3.0 Tissue: umbilical vein endothelium, LPS-stimulated Gene product: endothelin 1 [EDN1] Alleles: A1, B1, B2, A2, B3, B4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.9, 0.9; HindIII--4.0, 1.7; SspI--2.3, 2.3, 0.7, 0.3; AvaI--4.3, 1.4; BglI--4.5, 1.2. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.75; EcoRI--1.2; SspI--1.79, 2.04. Because of the internal EcoRI site, the insert cannot be excised intact. For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended. This insert contains two identical copies of 3' 0.9 kb EcoRI fragments. Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglII, EcoRI, HindIII, MspI, NruI, PstI, SstI, StyII, XbaI, and XhoI (16 unrelated individuals). |
| References |
Bloch KD, et al. Structural organization and chromosomal assignment of the gene encoding endothelin. J. Biol. Chem. 264: 10851-10857, 1989. PubMed: 2659594
Hoehe MR, et al. TaqI identifies a four allele DNA polymorphism of the human endothelin-1 gene (EDN1). Nucleic Acids Res. 19: 3161, 1991. PubMed: 2057379
Kenneth D Bloch, personal communication
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