Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.4; HindIII--4.5; PstI--2.1, 1.3, 0.8, 0.2; PvuII--4.5; XhoI--3.0, 1.0, 0.6. The insert contains the following restriction sites (nt position according to the revised sequence): BamHI--12; PstI--141, 336, 1093; XhoI--393; PvuII--979. Detects genomic EcoRI fragments of the following sizes (kb): human--12.0; Chinese hamster (CHO-K1 cell line)--9.0. When transfected into COS-7 cells or human cells from a galactosemic individual (GM639), enzyme activity was expressed. |
