| Vector |
Construct size (kb): 6.130000114440918 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI closer to XhoI site
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGB5
Genomic copy number: unique
Gene name: integrin, beta 5
Contains complete coding sequence?: Y
Tissue: thymus epithelium
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.174
Cross references: Insert lengths(kb): 3.174000024795532 Tissue: thymus epithelium Gene product: integrin, beta 5(integrin beta 5) [ITGB5] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 3.0; PstI--3.9, 1.0, 1.0, 0.2; KpnI--5.0, 0.9; PvuII--2.4, 2.1, 1.4; BamHI--6.0. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.64, 1.73, 1.92, 2.14; KpnI--0.92; PvuII--1.92. |
