| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--11.0,8.4,6.0,4.2,3.6,2.4,2.0; HindIII--18.0,5.0,4.0,3.4,2.8,2.1,1.3; BglII--15.0,6.8,5.2,4.2,2.9,1.7,1.3,1.1; SalI--39.0, 2.5; EcoRI--10.0,5.5,3.2,2.6,2.45,1.9,1.75. Codominant segregation of the MspI RFLP was observed in 45 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed after competitive hybridization with total human DNA. Enzyme(s) not detecting polymorphism: TaqI, RsaI, BglII, PstI and PvuII. |
