Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site. Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 0.6; PstI--5.0, 1.0; BamHI--uncut; SalI--6.0; HindIII--6.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The probe hybridizes to the inserted sequence of the p11-1-111 RFLP and thus fails to hybridize to chromosomes lacking this sequence. |
