Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet).
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References
Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831
