Restriction digests of the clone give the following sizes (kb): HindIII--10.05; XhoI--10.05; BglII--uncut. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The hygromycin resistance gene is expressed under the control of the HSV-TK promoter with a polyadenylation signal. This is not an expression vector. The cloning sites were derived from the M13mp10/pUC12 polylinker with the EcoRI site having been destroyed. |
