天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

Construct size (kb): 7.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: HindIII
Vector end: SmaI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC9A1
Other gene symbols: APNH; NHE1
Gene name: solute carrier family 9 (sodium/hydrogen exchanger),
isoform 1 (antiporter, Na+/H+, amiloride sensitive)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p36.1-p35
Tissue: kidney cortex
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: Modification: blunt ended
Insert size (kb): 4.0
Cross references: DNA Seq. Acc.: M81768

Insert lengths(kb): 4.0

Tissue: kidney cortex

Gene product: NA(+)/H(+) antiporter 1( Na+/H+ antiporter, Na+/H+ antiporter protein) [APNH]

Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.2, 3.1; BamHI--3.4, 2.0, 1.5, 0.41; PstI--7.2; SacI--7.2; PvuII--2.7, 2.45, 1.10, 0.56, 0.28.

Constructed from a 520 bp HindIII/PstI PCR product and the large PstI/EcoRI fragment from HK-3.5.

The SmaI site was lost during cloning, and the vector SacI site was deleted.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.38, 2.3, 3.7; PstI--0.52; SacI--1.1; PvuII--2.1, 2.6, 2.9, 3.9.