| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5, 3.5; PvuII--4.2, 3.4, 1.0, 0.6. The insert can transform NIH 3T3 cells. The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment. Contains pBR322 (EcoRI/PvuII, 2.05 kb); SV40 ori (PvuII/HindIII, 344 bp); exon 1 (HindIII/HindIII); exon 2 (HindIII/EcoRI, 760 bp); exon 3/exon 4 (EcoRI/SacI, 5 kb), and SV40 polyadenylation signal (SacI/EcoRI). |
