| 產品名稱 |
pCMV-SREBP2-468 |
| 商品貨號 |
B238791 |
| Designations |
pCMV-SREBP2-468 |
| GenBank Number |
NM_004599
|
| Species |
Homo sapiens, human |
| Depositors |
JD Horton, JL Goldstein |
| Applications |
contains sequence sterol regulatory element binding transcription factor 2 sterol regulatory element-binding protein 2 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.480000019073486 Tissue: HeLa cells Gene product: sterol regulatory element binding transcription factor 2(sterol regulatory element-binding protein 2) [SREBP2] Target Gene: sterol regulatory element binding transcription factor 2 |
| Insert Size (kb) |
1.480 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Frozen glycerol stock of E. coli containing the plasmid |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.4; SmaI--6.4; EcoRI/SmaI--4.6, 1.8; PstI--5.6, 0.8; BamHI/PstI--5.6, 0.8. Contains 75 bp of 5'UTR and sequence for amino acids 1-468 of human SREBR2. It is not full length but encodes the transcriptionally active form of the protein. Insert cannot be excised. |
| References |
Shimano H, et al. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J. Clin. Invest. 99: 846-854, 1997. PubMed: 9062341
Horton JD, et al. Activation of cholesterol synthesis in preference to fatty acid synthesis in liver and adipose tissue of transgenic mice overproducing sterol regulatory element-binding protein-2. J. Clin. Invest. 101: 2331-2339, 1998. PubMed: 9616204
|
