Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.45, 0.75; EcoRI--5.3; XhoI--3.0, 2.3; SacI--5.3; PstI--2.5, 1.5, 1.3. The insert contains the following restriction sites (bp from the 5' end of the cDNA sequence): BamHI--1290; EcoRI--274; HincII--1951; SacI--1010; PstI--1468; XbaI--138, 1570. The insert sequence is essentially identical to that reported for fibroblast collagenase except for noted changes. Contains the complete coding sequence (nt 68-1478 of the cDNA). The 2.2 kb XhoI fragment includes some sequence from the vector: 175 bp at the 5' end and 50 bp at the 3' end. |
