This is a YE-type (yeast episomal) vector with the TRP1 marker. The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9. |
