| Applications |
Prehybridization with human DNA is recommended. Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians). |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--22, 8 (arms), 9.4 (insert); EcoRI--22, 7.5, 6.2, 4.9, 2.4; BamHI/EcoRI--22, 7.5, 4.9, 4.3, 2.2, 1.8. The map location has been revised to 8p23-q11. Insert contains EcoRI (4.3 kb, 9.2 kb) sites (distance in kb from 23.58 arm). Prehybridization with human DNA is recommended. Codominant segregation of the HindIII polymorphism has been demonstrated in 6 families (29 individuals). Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians). Enzyme(s) not detecting polymorphism: ApaI, BclI, BglI, BglII, DraI, HgiAI, MboI, NsiI, RsaI, SacI, XhoI. |
| References |
Cottingham AW, et al. A new StyI RFLP and haplotypes with the HindIII RFLP at the D8S5 (TL11) locus. Nucleic Acids Res. 19: 4790, 1991. PubMed: 1679925
Dietzsch E, et al. An anonymous human single copy genomic clone (D8S5) (TL11) on chromosome 8 identifies a moderately frequent RFLP. Nucleic Acids Res. 14: 6781, 1986. PubMed: 3018683
|
