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pJCZ3.1 [RW-164]

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編號(hào):B237818

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產(chǎn)品名稱(chēng)	pJCZ3.1 [RW-164]
商品貨號(hào)	B237818
Designations	pJCZ3.1 [RW-164]
Species	Homo sapiens, human
Depositors	RL White, Y Nakamura
Applications	RFLPs observed under normal stringency (0.1X SSC).
Vector	Construct size (kb): 8.600000381469727
Insert	DNA: genomic Insert lengths(kb): 6.0 Gene product: DNA Segment, single copy [D19S20]
Insert Size (kb)	6.0
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): PstI--7.9, 0.71; EcoRI--8.6; HindIII--8.6; BamHI--4.3 doublet; HincII--7.6, 0.95; EcoRI/HindIII--6.0, 2.7; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pJCZ3.1. The hybridization pattern of this probe with human DNA was not checked by the ATCC. Subcloned from cosmid JCZ3 isolated by zeta globin oligonucleotide. RFLPs observed under normal stringency (0.1X SSC).
References	Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pJCZ3.1 on chromosome 19 [D19S20]. Nucleic Acids Res. 16: 1229, 1988. PubMed: 2894014 Peter J O'Connell, personal communication

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