| Vector |
Construct size (kb): 5.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens DNA Segment, single copy, expressed
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: T08901; EST: EST06793; Primer: M13-21; THC
id: THC83492
DNA Seq. Acc.: T08902; EST: EST06794; Primer: M13 Reverse
DNA Seq. Acc.: X61451
Nucleotides 1-60, 60-371 of the insert correspond to
nucleotides 281-340, 339-650 of X61451 with 94% identity. Insert lengths(kb): 2.099999904632568 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, single copy, expressed [F41*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; HindIII/NotI--3.3, 2.1. Based on the alignment of EST06794 with GenBank accession X61451 (mouse brain F41 transcript, 1040 nt, CDS 82-855), this clone appears to contain part of the coding region and 3' untranslated sequence. |
