Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
From pool of lung, liver, and spleen of domestic cat (neonate death)
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Antigenic cross-reactivity with feline coronavirus; feline infectious peritonitis, strain NOR 15; and canine coronavirus, Binn strain 1-71.
Antigenic cross-reactivity with feline coronavirus; feline infectious peritonitis, strain NOR 15; and canine coronavirus, Binn strain 1-71.
Effect on Host
Yes, in vitro effects: Cytopathic effects (multinucleated giant cells followed by rounding in 2 days)
CPE, multinucleated giant cells followed by rounding.
Recommended Host
CRFK cells (ATCC CCL-94)
Recommended Host: CRFK cells (ATCC? CCL-94™)
CRFK cells (ATCC CCL-94); cat
Growth Conditions
Temperature: 37°C
Incubation: 2 to 3 days at 37°C, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Name of Depositor
AJ McKeirnan, JF Evermann
Special Collection
ATCC
Source
From pool of lung, liver, and spleen of domestic cat (neonate death)
References
Pedersen NC, et al. Pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. Am. J. Vet. Res. 45: 2580-2585, 1984. PubMed: 6084432
McKeirnan AJ, et al. Isolation of Feline Coronaviruses from Two Cats with Diverse Disease Manifestations. Feline Pract. 11: 16-20, 1981.
