Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI/SstI--4.1, 2.7; EcoRI/HindIII--2.7 (doublet), 0.6, 0.45, 0.3, 0.25; KpnI--5.1, 1.7; SalI--3.3, (doublet), 0.25. The insert contains the following restriction sites (approximate kb from the 5' end): AccI--0.95; AvaI--1.0, 2.8; KpnI--1.6; SalI--0.95. A periplasmic enzyme preparation of transformed E. coli cells yields 1.7 units per ml of culture broth, compared with 0.4 units per ml of culture broth from the parent organism. The source strain is a gram-negative marine bacterium associated with the brown algae Sargassum fluitans. A 1.6 kb KpnI fragment detects the following genomic restriction fragments in the bacterial strain SFFB080483Alg-A (approximate sizes in kb): EcoRI--23; KpnI--1.8; HincII--2 fragments smaller than 1.8. |
