| Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--6.8, 4,8; SalI--12.0; BamHI--7.8, 3.6. The plasmid contains the following restriction sites (approximate kb from nt 1 of the GenBank record): BamHI--3.82, 4.13, 7.55; BglI--4.51, 5.98, 7.30, 10.46; ClaI--5.57, 8.23; EcoRI--0.00, 6.96; HindIII--5.49, 5.65, 7.45; KpnI--0.63, 3.45, 8.86; NdeI--5.99, 9.27; PstI--1.16, 1.33, 10.58; PvuI--1.01, 2.71, 10.71; SacI--0.56, 3.15, 8.79; SalI--4.30; SmaI--0.62, 5.10, 8.85; XbaI--0.44, 5.92, 8.67; XhoI--2.15, 6.92. AM-MLV (ATCC VR-1448) is an engineered broad host range helper virus. It is a fusion of amphotropic virus 4070A pol-env with Mo-MLV gag region. It can infect a wide range of mouse cell types (including BALB/c) as well as human and chicken cells. This AM-MLV clone can be used as a reference standard for detection of murine retroviruses. The Hybrid Amphotropic/Moloney Murine Leukemia Virus, pAMS, was constructed by inserting the 1541bp SalI-ClaI 4070A pol-env containing fragment into homologous region of Mo-MLV using the cloning vector pBR322 as a backbone. |
