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pPNP1

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編號:B235286

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產品名稱	pPNP1
商品貨號	B235286
Designations	pPNP1
GenBank Number	K02574
Species	Homo sapiens, human
Depositors	DW Martin
Vector	Construct size (kb): 6.0
Insert	DNA: cDNA Insert lengths(kb): 1.417999982833862 Tissue: HeLa cell line Gene product: nucleoside phosphorylase [NP]
Insert Size (kb)	1.418
Media	ATCC^® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.7, 0.58; BglII--6.0; EcoRV--4.4, 1.55; KpnI--6.0; BamHI--4.8, 1.2. Although there is a AATAAA sequence in the insert, is is 208 bp from the 3' end of the insert which does not contain poly(A). Thus this is probably not the authentic polyadenylation signal. Each end of the insert contains 20-22 dC/dG bp from the cloning procedure. The single long open reading frame encodes 289 amino acids, corresponding to a molecular weight of 32.1 kDa.
References	Osborne WR, Miller AD. Design of vectors for efficient expression of human purine nucleoside phosphorylase in skin fibroblasts from enzyme-deficient humans. Proc. Natl. Acad. Sci. USA 85: 6851-6855, 1988. PubMed: 3137573 Williams SR, et al. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. PubMed: 6087295 Goddard JM, et al. Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli. Proc. Natl. Acad. Sci. USA 80: 4281-4285, 1983. PubMed: 6410388 Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J. Biol. Chem. 262: 2332-2338, 1987. PubMed: 3029074

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