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Rotavirus A

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編號:B234635

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產品名稱	Rotavirus A
商品貨號	B234635
Classification	Reoviridae, Rotavirus, Rotavirus A
Deposited As	Human rotavirus
Agent	Rotavirus A
Strain	Wa (TC adapted)
Applications	Efficacy testing Testing Testing virucides Viruscide testing Enteric Research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen 1 mL per vial
Storage Conditions	-70°C or colder
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments	VP7 serotype 1 (G1).
Effect on Host	CPE, refractile rounding and sloughing.
Recommended Host	MA-104 Clone 1 (ATCC^® CRL-2378.1™)
Growth Conditions	Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere while continuously rocking. End adsorption by adding virus growth medium. Incubation: 1-7 days
Name of Depositor	RG Wyatt, National Institute of Allergy and Infectious Disease
U.S. Patent	4,341,870
U.S. Patent Number	4,341,870
Source	Diarrhea stool from patient positive for rotavirus
References	ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02. Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99. Wyatt RG, et al. Cultivatable human rotavirus type 2. US Patent 4,341,870 dated Jul 27 1982 Water supply -- Domestic type water treatment appliances -- Performance requirements. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4348:1995. Wyatt RG, James WD. Methods of gastroenteritis virus culture in vivo and in vitro In: Tyrrell AAJ, Kapikian ZA Viral infections of the gastrointestinal tract Marcel Dekker, New York, pp. 13-35, 1982 Wyatt RG, et al. Human rotavirus type 2: cultivation in vitro. Science 207: 189-191, 1980. PubMed: 6243190
Cross References	Nucleotide (GenBank) : AF200224 Human rotavirus strain Wa clone 1 nonstructural protein NSP4 mRNA, complete cds. Nucleotide (GenBank) : AF200225 Human rotavirus strain Wa clone 2 nonstructural protein NSP4 mRNA, complete cds.

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