| Insert |
DNA: genomic Insert lengths(kb): 7.099999904632568 Gene product: DNA Segment, single copy [DYS1] Alleles: B2, D4, A5, B1, C2, D3, D4, F2, I2, D2, A1, A2, A3, A4, C1, D1, F1, I1 |
| Comments |
This plasmid was constructed by linkering XbaI/Sau3A fragments from the Y chromosome with EcoRI and inserting into the EcoRI site of pJB8. There are EcoRI sites in the insert so the intact insert cannot be released by an EcoRI digest. Restriction digests of the clone give the following sizes (kb): BamHI--11.0; BamHI/XbaI--10.0, 0.94; HindIII--6.4, 4.7; SalI--12.5; EcoRI--5.4, 4.2, 0.66, 0.52. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The single-copy BamHI/XbaI fragment has been sequenced. The single-copy probe (0.9 kb) of the insert can be excised with a BamHI/XbaI double digest. It must be separated from highly repeated flanking sequence by gel electrophoresis. At low stringency (2X SSC, 42C) TaqI detects 4 Y-specific bands which are independently polymorphic and several other Y-specific constant bands. |
| References |
Ngo KY, et al. A DNA probe detecting multiple haplotypes of the human Y chromosome. Am. J. Hum. Genet. 38: 407-418, 1986. PubMed: 3010708
Spurdle A, Jenkins T. Y chromosome probe p49a detects complex PvuII haplotypes and many new TaqI haplotypes in southern African populations. Am. J. Hum. Genet. 50: 107-125, 1992. PubMed: 1729883
Lucotte G, et al. Nucleotide sequence of p49a, a genomic Y-specific probe with potential utilization in sex determination. Mol. Cell. Probes 5: 359-363, 1991. PubMed: 1791857
Jean Weissenbach, personal communication
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