| Vector |
Construct size (kb): 600.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pYAC-RC
Intact vector size: 11.700
Type of vector: YAC
Vector end: ClaI
Vector end: ClaI
Cloning sites: NotI SacII SalI mLuI ClaI SnaBI SmaI
Polylinker sites: NotI SacII SalI mLuI ClaI SnaBI SmaI
Construction: pYAC3, polylinker
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
centromere: CEN4
marker(s): ampR, HIS3, SUP4, TRP1, URA3
promoter: none
replicon: pMB1, ARS1
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DXS102
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Genome: Homo sapiens
Gene symbol: DXS820
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26
Genome: Homo sapiens
Gene symbol: MCF2
Genomic copy number: unique
Gene name: MCF.2 cell line derived transforming sequence
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Tissue: DNA from human/hamster cell line X3000.11
Type of DNA: genomic
Insert end: TaqI
Insert end: TaqI
Insert size (kb): 600.0
Cross references: Insert lengths(kb): 600.0 Tissue: DNA from human/hamster cell line X3000.11 Gene product: MCF.2 cell line derived transforming sequence(Oncogene MCF2 (oncogene DBL)) [F9] Alleles: A1, A2, D2, C1, D1, A1, B1, A2, B2, C2 |
| References |
Abidi FE, et al. Yeast artificial chromosomes containing human Xq24-Xq28 DNA: library construction and representation of probe sequences. Genomics 7: 363-376, 1990. PubMed: 2365355
Little RD, et al. Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26. Proc. Natl. Acad. Sci. USA 89: 177-181, 1992. PubMed: 1729687
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