| 產(chǎn)品名稱 |
TT |
| 商品貨號(hào) |
B233024 |
| Organism |
Homo sapiens, human |
| Tissue |
thyroid/medulla |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Age |
77 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Karyotype |
aneuploid with several markers; modal number = 40; range = 32 to 86
(. Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.) |
| Derivation |
The TT cell line was established by S.S. Leong, et al. from a specimen obtained by needle biopsy from a 77 year old female with thyroid medullary carcinoma. |
| Clinical Data |
77 years
Caucasian
female
|
| Genes Expressed |
calcitonin; carcinoembryonic antigen (CEA) |
| Cellular Products |
calcitonin; carcinoembryonic antigen (CEA) |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
TT cells continuously produce high levels of calcitonin and CEA.
Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively, after a medium change.
CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period.
Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.
The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine.
It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F12K medium.
Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,13 D13S317: 11 D16S539: 12,13 D5S818: 12,13 D7S820: 10,12 THO1: 6,9 TPOX: 8,11 vWA: 16,18 |
| Population Doubling Time |
83 hrs |
| Name of Depositor |
SS Leong |
| Deposited As |
Homo sapiens |
| Passage History |
The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine. |
| References |
. Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
Behr TM, et al. Improved treatment of medullary thyroid cancer in a nude mouse model by combined radioimmunochemotherapy: doxorubicin potentiates the therapeutic efficacy of radiolabeled antibodies in a radioresistant tumor type. Cancer Res. 57: 5309-5319, 1997. PubMed: 9393755
. Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
|
| Cross References |
Nucleotide (GenBank) :
AJ275978
Homo sapiens partial mRNA LAGE-2b for hypothetical protein (LAGE-2 gene).
|
