| Insert |
DNA: genomic Insert lengths(kb): 14.5 Tissue: liver Gene product: DNA Segment, single copy [D6S19] Alleles: C7, A1, A2, A3, C8, C1, C2, C3, C4, C5, C6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 12.0, 5.0, 4.6, 3.5, 1.4; BamHI--23.0, 15.0, 7.4, 5.6, 3.5, 1.4; HindIII-->23.0, 12.0, 5.4, 3.2, 0.76; BglI--23.0, 8.2 (doublet), 5.0, 1.9; PvuI-->23.0, 15.0, 4.8, 2.5, 1.9. The TaqI and MspI polymorphisms have a combined PIC value of 0.60 and a combined heterozygosity of 69%. Enzyme(s) not detecting polymorphism: BamHI, HindIII, EcoRI (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870
Knowlton RG, et al. Use of highly polymorphic DNA probes for genotypic analysis following bone marrow transplantation. Blood 68: 378-385, 1986. PubMed: 3524708
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