| Comments |
Restriction digests of the clone give the following sizes (kb, * = doublet): EcoRI--10.5, 10.1, 8.8, 5.4, 4.6*, 2.9, 2.5*, 1.0; XhoI--20.8, 10.7, 10.3; SalI--20.8*; BamHI--10.3*, 10.1*, 6.6, 3.5, 2.6, 1.8, 1.0; PstI--10.5, 6.2, 4.4*, 3.1 + more. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert contains Alu repeats, but the gene is single copy. p5T4-1-27 was isolated from a hamster line transformed with human lymphocyte/hamster hybrid DNA and pSV2gpt. The insert is a human gene with flanking human, pSV2gpt, and hamster sequences. p14B1 is 2.3 kb and contains ColE1 and ampR of pBR327, lambda cos sites, and a BamHI cloning site flanked by EcoRI sites 6 bp on either side. |
| References |
Weber CA, et al. Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells. Mol. Cell. Biol. 8: 1137-1146, 1988. PubMed: 2835663
Christine A Weber, personal communication
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