天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

Distribution host: Escherichia coli SCS1

Size (kb): 10.8000001907348600

DESCRIPTION OF VECTOR:
Intact vector size: 10.800
Type of vector: plasmid
Cloning sites: EcoRI SacI Ecl136II KpnI SmaI XmaI BamHI XbaI SalI PstI
HindIII
Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI
HincII PstI SphI HindIII
Construction: pMMB67EH (ATCC 37622), R100.1
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
promoter: tac, ->
MCS: EcoRI...HindIII, ->
transcription terminator: rrnB
restriction site: SphI
marker(s): strR (spcR)
replicon: oriV
replicon: oriT
replicon: RSF1010
repressor gene: lacIq

Vector: pJAK12 (plasmid)

Promoters: Promoter tac

Construction: pMMB67EH (ATCC 37622), R100.1

Marker(s):strR (spcR)

Construct size (kb): 10.80000019073486

Features: marker(s): strR (spcR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: EcoRI...HindIII
restriction site: SphI
transcription terminator: rrnB

expression vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--10.8; HindIII--10.8; SphI--9.7, 1.1.

A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.

Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.

Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.

Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.

Temperature: 37.0°C

component of:ATCC 87055