| Applications |
Enzyme(s) not detecting polymorphism: BglII, PvuII, TaqI, PstI, BstXI, XmnI, XbaI, StuI, BclI, NcoI, NdeI, BstNI, HphI, HindIII (tested against 4 American Caucasians). |
| Insert |
DNA: genomic Insert lengths(kb): 1.850000023841858 Tissue: neuroblastoma IMR-32 cell line Gene product: DNA Segment, single copy [D2S5] Alleles: A1, A2, B1, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.8; EcoRI--6.1; BamHI--6.1; SalI--6.1; PstI--3.7, 1.0, 0.7, 0.6; EcoRI/BamHI--4.3, 2.0; EcoRI/SalI--3.8, 2.3. Enzyme(s) not detecting polymorphism: BglII, PvuII, TaqI, PstI, BstXI, XmnI, XbaI, StuI, BclI, NcoI, NdeI, BstNI, HphI, HindIII (tested against 4 American Caucasians). |
| References |
Shiloh Y, et al. Two RFLPs identified by a human chromosome #2 clone at 2p15-2p16 (HGM8 assignment no. D2S5). Nucleic Acids Res. 13: 5403, 1985. PubMed: 4022784
Kanda N, et al. Isolation of amplified DNA sequences from IMR-32 human neuroblastoma cells: Facilitation by fluorescence-activated flow sorting of metaphase chromosomes. Proc. Natl. Acad. Sci. USA 80: 4069-4073, 1983. PubMed: 6575396
Shiloh Y, et al. Differential amplification, assembly, and relocation of multiple DNA sequences in human neuroblastomas and neuroblastoma cell lines. Proc. Natl. Acad. Sci. USA 82: 3761-3765, 1985. PubMed: 3858848
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