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Gallid herpesvirus 2

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編號:B226837

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產(chǎn)品名稱	Gallid herpesvirus 2
商品貨號	B226837
Classification	Herpesviridae, Mardivirus
Deposited As	Marek's disease virus
Agent	Gallid herpesvirus 2
Strain	GA-5
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	chicken tissue with Marek's disease
Product Format	frozen 1 mL per vial
Storage Conditions	Vapor phase of liquid nitrogen
Comments	Infected CK cell suspension with 7% DMSO + Penicillin/Streptomycin + Fungizone^?. Similar to JM strain. Dilution of infected chicken kidney cell suspension with medium 2-3 times the volume after thawing will enhance virus recovery.
Effect on Host	CPE, focal lesions
Recommended Host	chicken kidney cells
Growth Conditions	Temperature: 38.5°C Incubation: 5-7 days
Name of Depositor	BW Calnek, KA Schat, Cornell University New York State College of Veterinary Medicine
U.S. Patent	3,783,098
U.S. Patent Number	3,783,098
Source	Chicken tissue with Marek?s disease, Georgia, 1964
References	Calnek BW, Hitchner SB. Highly potent, viable and stable cell-free virus preparations from cells infected with cell-associated viruses and method for obtaining the same. US Patent 3,783,098 dated Jan 1 1974 Eidson CS, Schmittle SC. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 12: 467-476, 1968. PubMed: 4301071 Reddy SM, et al. Development of a quantitative-competitive polymerase chain reaction assay for serotype 1 Marek's disease virus. Avian Dis. 44: 770-775, 2000. PubMed: 11195630. Silva RF. Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Dis. 36: 521-528, 1992. PubMed: 1329706
Cross References	Nucleotide (GenBank) : U63464 Gallid herpesvirus 2 DNA polymerase gene, partial cds.

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