| 產(chǎn)品名稱 | UWB1.289+BRCA1 |
|---|---|
| 商品貨號 | B224800 |
| Organism | Homo sapiens, human |
| Tissue | ovary |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain CMV and SV40 vial DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | ovarian carcinoma |
| Age | 56 |
| Gender | female |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | UWB1.289+BRCA1 is a stable cell line derived from UWB1.289 (ATCC CRL-2945), a BRCA1-null human ovarian cancer line, in which wild-type BRCA1 was restored. |
| Clinical Data | female |
| Antigen Expression | BRCA1, positive cytokeratin 7 (CK-7), positive calretinin, positive Wilms' tumor protein (WT), positive |
| Receptor Expression | estrogen, not expressed progesterone, not expressed |
| Oncogene | p53 |
| Genes Expressed | p53, cytokeratin 7 (CK-7) |
| Comments | A pcDNA3 plasmid carrying wild-type BRCA1 was transfected into the parent line. Restoration of wild-type BRCA1 function in these cells partially restores DNA damage responses. Ref Restoration of wild-type BRCA1 function in these cells partially restores DNA damage responses. |
| Complete Growth Medium | The base medium for this cell line is:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended. Medium renewal: Every 2 to 3 days
|
| Cryopreservation | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: 5% CO2 in air recommended
Temperature: 37°C |
| STR Profile | D5S818: 13 D13S317: 9 D7S820: 7, 10 D16S539: 12 vWA: 16, 19 THO1: 9 TPOX: 9, 11 CSF1PO: 11 Amelogenin: X |
| Population Doubling Time | approximately 36 hours |
| Name of Depositor | E Swisher |
| Year of Origin | 2003 |
| References | DelloRusso, C., et al. Functional characterization of a novel BRCA1-null ovarian cancer cell line in response to ionizing radiation. Mol Cancer Res.;5(1):35-45, 2007. PubMed: 17259345 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
DelloRusso, C., et al. Functional characterization of a novel BRCA1-null ovarian cancer cell line in response to ionizing radiation. Mol Cancer Res.;5(1):35-45, 2007. PubMed: 17259345