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pcJE-1

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編號:B222750

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產品名稱	pcJE-1
商品貨號	B222750
Designations	pcJE-1
GenBank Number	M19681
Species	Mus musculus, mouse
Depositors	CD Stiles
Applications	Frequently used as a molecular marker for response to cellular growth factors. Has been used to identify the human homolog from WI-38 cells.
Vector	Construct size (kb): 3.5
Insert	DNA: cDNA Insert lengths(kb): 0.6000000238418579 Tissue: 3T3 (clone A31) cell line Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [Scya2]
Insert Size (kb)	0.600
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 0.63; BamHI--uncut; consistent with deposited DNA. Transcription from the SP6 promoter yields sense RNA. JE is one of several (including KC, JB) competence genes which are induced with the addition of platelet-derived growth factor. Frequently used as a molecular marker for response to cellular growth factors. Has been used to identify the human homolog from WI-38 cells. Includes a single open reading frame that presumably encodes a protein of 148 aa, with a consensus sequence for N-glycosylation at aa 126. The 3' region contains an ATTTA sequence described for cytokines and mRNAs with rapid turnover.
Classification	Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References	Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001 Rollins BJ, et al. Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties. Proc. Natl. Acad. Sci. USA 85: 3738-3742, 1988. PubMed: 3287374 Timmers HT, et al. E1A products of adenoviruses reduce the expression of cellular proliferation-associated genes. Oncogene Res. 3: 67-76, 1988. PubMed: 2974528 Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Wilson SD, Dorf ME. Localization of five cytokine genes to chromosome 11. Mouse News Lett. 85: 14-15, 1989.

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