| 產(chǎn)品名稱 |
Miamiensis sp. |
| 商品貨號 |
B222107 |
| Deposited As |
Miamiensis avidus Thompson and Moewus |
| Strain Designations |
Ma |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
test tube |
| Storage Conditions |
Test Tube: See handling procedure |
| Axenic/Xenic |
Axenic |
| Type Strain |
no |
| Comments |
Prey-induced transformation |
| Medium |
ATCC® Medium 1651: MA medium
|
| Growth Conditions |
Temperature: 25°C |
| Cryopreservation |
- Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
- Adjust the concentration of cells to 2 x 106 - 2 x 107/ml in fresh medium.
- While cells are centrifuging prepare a 22% (v/v) solution of sterile DMSO in fresh medium.
- Mix the cell preparation and the cyroprotectant in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 11% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
- Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
- The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
|
| Name of Depositor |
AT Soldo, EB Small |
| Chain of Custody |
ATCC <-- AT Soldo, EB Small <-- G. Holz |
| References |
. . J. Protozool. 19: 519-524, 1972.
Gomez-Saladin E, Small E. Prey induced transformation of Miamiensis avidus strain Ma/2 by a soluble factor. J. Eukaryot. Microbiol. 40: 550-556, 1993.
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