| 產(chǎn)品名稱 |
pRH512 |
| 商品貨號(hào) |
B220958 |
| Designations |
pRH512 |
| GenBank Number |
M77182
|
| Species |
Amsacta moorei entomopoxvirus |
| Depositors |
Univ. Florida Res. Fndn., Inc., RW Moyer, Univ. Florida Res. Fndn., Inc. |
| Applications |
The Entomopoxvirus spheroidin and thymidine kinase genes make attractive expression cassettes for heterologous genes which can be used in vertebrate poxviruses such as vaccinia and swine pox virus. |
| Vector |
Construct size (kb): 7.203999996185303 |
| Insert |
DNA: genomic Insert lengths(kb): 4.504000186920166 Gene product: spheroidin |
| Insert Size (kb) |
4.504 |
| Media |
ATCC® Medium 2000: 2X YT medium (1320)
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--4.5, 2.7; EcoRI--7.3; HindIII--6.4, 0.9. AmEPV is one of the few insect poxviruses which replicate in insect cell lines. AmEPV is unable to replicate in vertebrate cell lines. The Entomopoxvirus spheroidin and thymidine kinase genes make attractive expression cassettes for heterologous genes which can be used in vertebrate poxviruses such as vaccinia and swine pox virus. |
| Classification |
Poxviridae, Entomopoxvirinae, Betaentomopoxvirus |
| References |
Moyer RW, Hall RL. Entomopoxvirus spheroidin gene sequences. US Patent 5,476,781 dated Dec 19 1995
Hall RL, Moyer RW. Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames. J. Virol. 65: 6516-6527, 1991. PubMed: 1942245
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
