天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 4.7109999656677250

DESCRIPTION OF VECTOR:
Intact vector size: 4.711
Type of vector: plasmid
Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI
Polylinker sites: HindIII SalI BamHI [tk-promoter luc+ SV40 poly (A)] ClaI
SmaI KpnI SacI
Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI
XmnI SspI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: TK, 37-200
reporter group: luciferase (luc+), ->, 238-1890
terminator: SV40 late polyadenylation, 1922-2143
MCS: ClaI...SacI, 2146-2168
replicon: pMB1, 2587-2587
marker(s): ampR, <-, 3345-4205
terminator: SV40 large T-antigen polyadenylation, 4337-4708

Vector: ptkLUC+ (plasmid)

Promoters: Promoter TK

Marker(s):ampR

Construct size (kb): 4.710999965667725

Features: marker(s): ampR
promoter: TK
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...SacI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation

reporter construct

 shuttle vector

Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7.

The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.

The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.

The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.

The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.

Temperature: 37.0°C

component of:ATCC 87654