| 產品名稱 | HSAEC1-KT |
|---|---|
| 商品貨號 | B217761 |
| Organism | Homo sapiens, human |
| Tissue | lung, small airway |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial, packed cuboidal |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells immortalized by CDK4 and hTERT and contain SV40 promoter sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 22 |
| Gender | male |
| Applications | The immortalized HSAEC1-KT cells show a stable epithelial morphology and differentiated cytokeratin isoforms after over 100 population doublings, express the stem cell marker p63 and high levels of p16INK4a, and have an intact p53 checkpoint pathway (Ref
The HSAEC1-KT cells also exhibit characteristics of normal cells such as contact inhibition of growth and failure to form soft agar colonies or form tumors in immune compromised mice (Ref
TGFβ-treated HSAEC1-KT cells showed an elongated shape with markedly induced F-actin staining. This morphological change of enhanced front-rear polarity and cytoskeletal actin rearrangement are characteristic morphological changes of EMT (epithelial mesenchymal transition) (Ref |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Cytogenetic analysis was performed on G-banded metaphase cells from the human cell line HSAEC1-KT. Several abnormal male near-diploid karyotypes are found: Clone 1 demonstrates trisomy 5 with no other aberrations. 47,XY,+5 Clone 2 demonstrates trisomy 5 and 20 with no other aberrations. 48,XY,+5,+20 Clone 3 demonstrates trisomy 5, an isochromosome of the long-arm of chromosome 10, resulting in three copies of the chromosome 10 long-arm and only one copy of the short-arm, and trisomy 20. 48,XY,+5,i(10)(p10),+20 Clone 4 demonstrates a deletion on the short-arm of chromosome 10 at band p10, a deletion on the short-arm of chromosome 17 at band p13, and trisomy 20. 47,XY,del(10)(p10),del(17)(p13),+20. |
| Images | 
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| Derivation | The HSAEC1-KT cell line was established by infecting primary human small airway epithelial cell culture with human telomerase (hTERT) and mouse cyclin dependent kinase 4 (CDK4) expressing retrovirus constructs and selecting under 250 ng/mL puromycin and 30 ug/mL G418 as described in PMID: 15604268 (Ref |
| Clinical Data | male 22 years |
| Antigen Expression | Positive for p63 (TP63) and Clara cell 10 (CC10) protein |
| Complete Growth Medium | SAGM BulletKit medium (Lonza CC-3119 and CC-4124)
To make the complete culture medium, add SAGM™ SingleQuots™ (Lonza CC-4124) which contains supplements and growth factors (BPE, Hydrocortisone, hEGF, Epinephrine, Transferrin, Insulin, Retinoic Acid, Triiodothyronine, BSA-FAF) to 500 mL bottle of SABM Basal Medium™, phenol red free basal medium (Lonza CC-3119) |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent.
|
| Cryopreservation | 80% complete growth media, 10% DMSO, 10% FBS |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | D5S818: 12 D13S317: 11, 12 D7S820: 8, 10 D16S539: 10, 11 vWA: 15, 18 Amelogenin: X, Y TPOX: 7, 8 CSF1PO: 11, 13 TH01: 7 |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | Boning Gao, Chunxian Huang, John Minna |
| References | Ramirez RD, Sheridan S, Girard L, et al. Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins. Cancer Res. 64(24):9027-9034, 2004. PubMed: 15604268 Kalita M, et al. Systems approaches to modeling chronic mucosal inflammation. Biomed. Res. Int. 2013: 505864, 2013. PubMed: 24228254 Gazdar AF, Gao B, Minna JD. Lung cancer cell lines: Useless artifacts or invaluable tools for medical science? Lung Cancer 68(3): 309-318, 2010. PubMed: 20079948 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
Ramirez RD, Sheridan S, Girard L, et al. Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins. Cancer Res. 64(24):9027-9034, 2004. PubMed: 15604268