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Hubs-5B [Hs1]

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編號:B217401

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產品名稱	Hubs-5B [Hs1]
商品貨號	B217401
Designations	Hubs-5B [Hs1]
GenBank Number	M30773
Species	Homo sapiens, human
Vector	Construct size (kb): 45.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lambdagt11 Intact vector size: 43.700 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Cloning capacity: 7.2 kb Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5) Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ promoter: lac replicon: lambda repressor gene: cI857 Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PPP3R* Genomic copy number: unique Gene name: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) Contains complete coding sequence?: Y Chromosome: 2; Localization: 2 Tissue: brainstem Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.8 Cross references: DNA Seq. Acc.: M30773 Nucleotides 1-1836 of the insert correspond to nucleotides 713-2548 of M30773. Insert lengths(kb): 1.799999952316284 Tissue: brainstem Gene product: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) [PPP3R*]
Insert Size (kb)	1.800
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0, 0.35, 0.28 (doublet); BamHI--16.0, 11.0, 6.5 (doublet), 5.6; BglII--19.0, 15.0, 7.0, 2.4, 0.7; EcoRV--5.6, 5.3, 4.5 (doublet), 3.7 (doublet), 3.3, 3.1, 2.5, 1.5, 1.4 (doublet), 1.3, 0.75, 0.6 (doublet); HindIII--21.0, 9.4, 6.0, 4.4, 3.8, 0.6; NdeI--20.0, 5.8 (doublet), 3.8, 2.4, 2.2, 1.7, 1.6, 0.55. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.61; EcoRI--0.27, 0.50, 1.57; EcoRV--0.53, 1.51; HincII--1.12; HindIII--0.13; NdeI--0.87; SphI--1.76. Corresponds to nt 804-2548 of the GenBank record. It does not include the complete coding sequence (nt 759-1271). Detects a 2.8-2.9 kb mRNA in rabbit brain, and a slightly smaller transcript in murine muscle and kidney.
References	Guerini D, et al. Isolation and sequence of a cDNA clone for human calcineurin B, the calcium-binding subunit of the Ca(2+)/calmodulin-stimulated protein phosphatase. DNA 8: 675-682, 1989. PubMed: 2558868

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