| 產品名稱 |
pCDM8-CD97 [CD97 cDNA] |
| 商品貨號 |
B217204 |
| Designations |
pCDM8-CD97 [CD97 cDNA] |
| GenBank Number |
X84700
|
| Species |
Homo sapiens, human |
| Depositors |
J Hamann |
| Applications |
in another host, produces protein CD97 antigen |
| Vector |
Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCDM8
Intact vector size: 4.356
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII XbaI XhoI BstXI PstI NotI
Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI
Construction: CMV, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1, 1-587
replicon: M13, <-, 588-1182
marker(s): supF, <-, 1183-1384
promoter: CMV, ->, 1516-2170
promoter for in vitro transcription: T7, ->, 2171-2189
MCS: HindIII...XbaI, ->, 2198-2655
terminator: SV40 splice site and polyadenylation, ->, 2656-3352
replicon: polyoma, 3353-4188
replicon: SV40, 4189-4352
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD97
Gene name: CD97 antigen
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 p13.12-13.2
Tissue: peripheral blood mononuclear cells, PMA-stimulated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.914
Cross references: DNA Seq. Acc.: X84700
Nucleotides 1-2914 of the insert correspond to
nucleotides 8-2921 of X84700. Insert lengths(kb): 2.914000034332275 Tissue: peripheral blood mononuclear cells, PMA-stimulated Gene product: CD97 antigen [CD97] Target Gene: CD97 antigen |
| Insert Size (kb) |
2.914 |
| Media |
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--4.1, 2.9; SmaI--5.1, 1.9; NcoI--2.7, 1.9, 0.85 (doublet), 0.8. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.80, 0.96; EcoRI--2.22; EcoRV--0.79; NcoI--0.06, 2.72; NdeI--2.28; PstI--2.11, 2.65; PvuI--2.07; PvuII--0.48, 0.95; SacI--1.69; SmaI--0.35, 2.26. |
| References |
Hamann J, et al. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain. J. Immunol. 155: 1942-1950, 1995. PubMed: 7636245
|
