Restriction digests of the clone give the following sizes (kb): BamHI--3.7; BglI--1.85, 1.2, 0.60; HindIII--3.7; PstI--3.3, 0.33; PvuII--2.4, 0.66, 0.50; EcoRI--2.7, 0.9. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.15; HindIII--0.69; PstI--0.04; PvuII--0.02; StuI--0.29, 0.39. Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies. It binds DNA. Contains the coding sequence for amino acids 109-374. The recombinant fusion protein contains an additional 23 N-terminal amino acids and additional C-terminal amino acids. |
