天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

in another host, produces protein gonadotropin-releasing hormone receptor

Construct size (kb): 5.559999942779541

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I
Intact vector size: 4.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI PstI EcoRV NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI
NsiI XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
promoter: CMV, ->
promoter: T7, ->
MCS: HindIII...XbaI, ->
promoter: SP6, <-
terminator: SV40 polyadenylation, ->
replicon: polyoma ori
replicon: SV40
replicon: pMB1
replicon: M13 ori, <-
marker(s): supF, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GRHR
Genomic copy number: unique
Gene name: gonadotropin-releasing hormone receptor
Contains complete coding sequence?: Y
Tissue: pituitary
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.56
Cross references: DNA Seq. Acc.: L03380

Insert lengths(kb): 1.559999942779541

Tissue: pituitary

Gene product: gonadotropin-releasing hormone receptor [GRHR]

Target Gene: gonadotropin-releasing hormone receptor

Restriction digest of the clone give the following sizes (kb): EcoRI--4.0, 1.55; BamHI--4.95, 0.6; HindIII--4.3, 0.8, 0.4; PstI--4.65, 0.9; PvuII--2.2, 1.8, 1.55.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.55; HindIII--0.42, 1.26; NdeI--1.06; NcoI--0.53; PstI--0.65; PvuII--0.70; SphI--1.42.

Contains the complete coding sequence (nt 56-1042).

The following primers can be used to amplify an 800 bp DNA fragment contained in the gene: upstream 5'-GCTTGAAGCTCTGTCCTGGGA-3'; downstream 5'-CCTAGGACATAGTAGGG-3'.

When transfected into COS-7 cells, expresses a high-affinity receptor.

Detects a 5.0 kb RNA transcript in human pituitary.

Transcription from the T7 promoter gives the sense strand.