Insert contains the following restriction sites (in bp from the 5' end): BclI--99; BglII--964; HincII--223; PstI--472; ScaI--1336; SmaI--1172; XhoI--1332. Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.6; PstI--2.75, 1.25, 0.55; SmaI--4.6. Contains a complete coding sequence, with 5' and 3' untranslated regions. The open reading frame is 1014 bp. At the amino acid level, there are 6 amino acid changes between the bovine and human sequences. |
