| Vector Information |
Size (kb): 6.0000000000000000 Vector: pLES2 (plasmid) Construction: pGC3, pUC9 lacZ Marker(s):ampR Construct size (kb): 6.0 Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1, pSY1005 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SmaI--6.0; HindIII--2.4, 3.6; EcoRI--6.0; AvaI--4.0, 2.1. Contrary to the diagram in the reference, there are two AvaI sites on the vector. The beta-lactamase production phenotype functions in both Escherichia coli and N. gonorrhoeae. An Escherichia coli/Neisseria gonorrhoeae shuttle vector. |
| References |
Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879
Stein DC, et al. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 25: 241-247, 1983. PubMed: 6319238
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