| 產品名稱 |
pDP122B |
| 商品貨號 |
B213965 |
| Designations |
pDP122B |
| Species |
Influenza A virus |
| Depositors |
Health Research, Inc., D Panicali, Health Research, Inc. |
| Vector |
Construct size (kb): 13.30000019073486 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: influenza virus
Gene name: hemagglutinin
Contains complete coding sequence?: U
Strain: A/PR/8/34
Type of DNA: cDNA
Insert end: PstI
Insert end: PstI
Insert size (kb): 7.3
Cross references: Insert lengths(kb): 7.300000190734863 Gene product: hemagglutinin |
| Insert Size (kb) |
7.300 |
| Media |
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--5.3, 4.1, 3.75; EcoRI--7.7, 3.9, 2.45; BamHI--12.0, 1.55. The order of fragments is as follows: PstI-BamHI (vaccinia)--2 kb; BamHI-BamHI (influenza)--1.78 kb; BamHI-PstI (vaccinia)--3.5 kb. The 2.0 kb BamHI fragment also contains an AvaI site and an EcoRI site. This was constructed by inserting a 5.5 kb PstI fragment from vaccinia into pBR325 (modified to remove the BamHI site). A 1.8 kb HindIII fragment encoding hemagglutinin was linkered with BamHI and inserted into a BamHI site of the vaccinia insert. |
| Classification |
Orthomyxoviridae, Influenzavirus A |
| References |
Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988
Dennis Panicali, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
