Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18. It includes three of sixteen Ki-67 repeats. Detects splicing isoforms of 8.7 and 9.8 kb. |
