Restriction digests of the clone give the following sizes (kb): EcoRI--7.5; BamHI--7.5; HindIII--5.1, 2.2, 0.2. Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences. One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number. Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423. |
