| 產品名稱 |
379-10 |
| 商品貨號 |
B213271 |
| Designations |
379-10 |
| Species |
Homo sapiens, human |
| Depositors |
LM Kunkel |
| Applications |
The 0.6 and 0.5 kb fragments are unique in the genome and can be used as a hybridization probe to detect a 5 kb mRNA (3' end). |
| Vector |
Construct size (kb): 9.800000190734863 |
| Insert |
DNA: genomic Insert lengths(kb): 5.400000095367432 Gene product: cytochrome b-245, beta polypeptide (chronic granulomatous disease)(cytochrome b-245, beta polypeptide) [CGD] |
| Insert Size (kb) |
5.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--9.8; PvuII--4.4, 3.6, 1.7; EcoRI--7.1, 2.7; BamHI--9.8, HindIII--5.4, 4.4. Digestion with BglII and HindIII together release 4 DNA fragments of 2.5, 1.8, 0.6, and 0.5 kb. The 0.6 and 0.5 kb fragments are unique in the genome and can be used as a hybridization probe to detect a 5 kb mRNA (3' end). |
| References |
Royer-Pokora B, et al. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature 322: 32-38, 1986. PubMed: 2425263
Monaco AP, et al. Detection of deletions spanning the Duchenne muscular dystrophy locus using a tightly linked DNA segment. Nature 316: 842-845, 1985. PubMed: 2993910
|
