| 產品名稱 |
DT201 [pDT201] |
| 商品貨號 |
B212923 |
| Designations |
DT201 [pDT201] |
| GenBank Number |
K01722
|
| Species |
Corynebacterium diphtheriae (Kruse) Lehmann and Neumann |
| Depositors |
President and Fellows of Harvard College, JR Murphy, President and Fellows of Harvard College |
| Vector |
Construct size (kb): 3.5
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
|
| Insert |
Genome: Corynebacterium diphtheriae
Gene name: diphtheria toxin, fragment A
Contains complete coding sequence?: N
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 0.831
Cross references: DNA Seq. Acc.: K01722 Nucleotides 1-831 of the insert correspond to nucleotides 58-889 of K01722.
Insert lengths(kb): 0.831
Gene product: diphtheria toxin, fragment A |
| Insert Size (kb) |
0.831 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Plasmid made from pUC8 and a small segment of the Corynebacteriophage beta tox-45 genome.Insert corresponds to nucleotides 57-888 of the genbank sequence K01722. |
| References |
Leong D, et al. Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220: 515-517, 1983. PubMed: 6403984
Murphy JR. Hybrid protein. US Patent 4,675,382 dated Jun 23 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
