| 產(chǎn)品名稱 |
BK-4E |
| 商品貨號(hào) |
B212417 |
| Designations |
BK-4E |
| Species |
eukaryotic cells |
| Depositors |
Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky |
| Applications |
in suitable host, produces protein eukaryotic translation initiation factor 4E |
| Vector |
Construct size (kb): 12.80000019073486 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: BK shuttle
Intact vector size: 11.414
Type of vector: phagemid
Vector end: XhoI
Vector end: BamHI
Cloning sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Polylinker sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Construction: RDB-0
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: T/t-Ag, <-
replicon: BK papovavirus
marker(s): neoR, <-
promoter: SV40
enhancer: dioxin responsive cytochrome P-450
promoter for expression: MMTV, ->
promoter for in vitro transcription: SP6, ->
MCS: AatII/.../SacII, ->
promoter for in vitro transcription: T7, <-
replicon: f1, <-
marker(s): ampR, ->
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: eukaryotic cells
Gene symbol: EIF4E
Other gene symbols: EIF-4E
Gene name: eukaryotic translation initiation factor 4E
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: BamHI linker
Insert size (kb): 1.4
Cross references: Insert lengths(kb): 1.399999976158142 Gene product: eukaryotic translation initiation factor 4E [EIF4E] Target Gene: eukaryotic translation initiation factor 4E |
| Insert Size (kb) |
1.400 |
| Media |
LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.5, 1.4; BamHI--13.0; EcoRI--13.0; PstI--7.5, 3.8, 1.0; XhoI--13.0. |
| References |
De Benedetti A, Rhoads RE. Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrrant growth and morphology. Proc. Natl. Acad. Sci. USA 87: 8212-8216, 1990. PubMed: 2122455
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
