| Comments |
Restriction digests of the clone give the following sizes (kb): NotI--34.0, 9.6; SalI--34.0, 9.6; EcoRI--19.0, 16.0, 5.6, 4.8, 1.75; HindIII--20.0, 11.0, 6.6, 4.8, 2.6, 0.9; HindIII/SacI--18.0, 10.0, 4.4, 3.2, 2.6, 2.2, 1.25, 1.2, 1.15, 1.05, 0.9, 0.7, 0.5; SacI--20.0, 9.4, 4.8, 1.7, 1.25, 1.2, 1.15, 0.9; HindIII/HincII--6.0, 5.6, 4.8, 3.3 (doublet), 2.5, 2.3 (doublet), 2.05, 1.75, 1.4, 1.2, 1.1, 0.9, 0.6. An 800 bp HincII/HindIII fragment from the 3' end of the coding region and 3' untranslated sequence has been used as a specific probe. Detects human genomic restriction fragments of the following sizes (approximate kb): EcoRI--9.5; HindIII--4.7; PstI--3.8. Contains the complete protein coding region, which consists of a single exon. The GenBank record corresponds to the 2.4 kb SacI/HindIII partial digest fragment 0.7 kb from the 3' end of the insert. Detects human mRNA of 9.5 kb (major) and 4.4 and 2.6 kb (minor). |
