Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 2.7; HindIII--8.7; KpnI--7.3, 1.35; EcoRI--4.9, 2.0, 0.64 (doublet), 0.5; PstI--4.4, 1.8, 1.7, 0.54. Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2. Encodes aa 160-910, including the DNA-binding domain and the hormone-binding domain. Also encodes 16 Gly between aa 447 and 464. Cross hybridizes with a 6.8 kb rat liver mRNA. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. |
